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KMID : 1149220120200020065
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Korean Journal of Oriental Medical Prescription
2012 Volume.20 No. 2 p.65 ~ p.82
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Anti-oxidative and anti-inflammatory effects of Odukhwan and Sasinhwan in RAW264.7 cells
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Kim Tae-Hoon
Cho Hyun-Jin
Park Sun-Dong
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Abstract
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Objectives : The aim of this study was verification of the anti-oxidative and anti-inflammatory effects of Odukhwan(ODH) and Sasinhwan(SSH) in mouse macrophage, RAW264.7 cells.
Methods : To investigate the anti-oxidative effect and scavenging activities of DPPH radical, superoxide anions, nitric oxide and peroxynitrite were measured. Cytotoxic activity of extract of ODH and SSH on RAW264.7 cells was measured using MTS assay. To proof the reductive activity of intracellular oxidation, DCFH-DA assay was performed. The nitric oxide(NO) production was measured and pro-inflammatory cytokines and PGE2 were measured by ELISA kit. The levels of inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2) and nuclear NF-κB p65 expression were detected by western blot.
Results : After those analyses, we bring to a conclusion as follows. Both herbal formulations scavenged DPPH radical and nitric oxide. But ODH had no scavenging activity of superoxide anions and SSH had low scavenging activity in peroxynitrite. And the results indicated that ODH and SSH inhibited the LPS-induced NO, PGE2 production
and iNOS, COX-2 expression accompanied by an attenuation of IL-1β and IL-6 production in RAW264.7 cells. They also have suppression effects of LPS-induced NF-κB activation.
Conclusions : ODH and SSH have anti-oxidative and anti-inflammatory effects and they may be a part of database for development of new anti-oxidative and anti-inflammatory drugs
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KEYWORD
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Anti-oxidation, Anti-inflammation, Herbal prescription, RAW264.7
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